Journal: Neuron

Article Title: Discrete evaluative and premotor circuits enable vocal learning in songbirds

doi: 10.1016/j.neuron.2019.07.025

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse anti-GFP Invitrogen A-11122 mouse anti-TH Invitrogen MA1–24654 rabbit anti-mCherry Abcam {"type":"entrez-nucleotide","attrs":{"text":"AB167453","term_id":"45421876","term_text":"AB167453"}} AB167453 rabbit anti-PV Abcam AB11427 rabbit anti-TH Millipore AB152 chicken anti-GFP Abcam AB13970 donkey anti-chicken Jackson ImmunoResearch 703–545–155 goat anti-rabbit Invitrogen A31556, A11037 goat anti-mouse Invitrogen A11001 Bacterial and Virus Strains AAV1-CAG-ChR2(H134R)-mCherry Penn Vector Core, This manuscript Addgene 100054-AAV1 AAV9-CAG-NRXN-ChR2-YFP Penn Vector Core, In House scAAV9-CMV-GFP UNC Vector Core AAV9-CAG-ChR2(H134R)-mCherry Penn Vector Core Chemicals, Peptides, and Recombinant Proteins Cholera Toxin Subunit B 647 Invitrogen Cat# 34778 Dextran, Alexa Fluor 488 Invitrogen Cat# {"type":"entrez-nucleotide","attrs":{"text":"D22910","term_id":"426835","term_text":"D22910"}} D22910 C&B Metabond Parkell S380 Low Toxicity Silicone Adhesive World Precision Instruments KWIK-SIL Fluoromount-G Southern Biotech Cat# 0100–01 Blocking One Histo Nacalai Tesque 06349–64 Experimental Models: Organisms/Strains Zebra Finches (Taeniopygia guttata) Recombinant DNA Zebra Finch VGLUT2 cDNA GenScript Zebra Finch VGAT cDNA GenScript Software and Algorithms MATLAB Mathworks https://www.mathworks.com/ Python Python Software Foundation https://www.python.org/ Labview National Instruments http://www.ni.com/enus/shop/labview.html Custom-written Labview software (EvTaf) Michael Brainard Lab ImageJ NIH https://imagej.nih.gov/ij/index.html Other Fiber Optic Fiber Thor Labs Cat# TS1843490 Fiber Optic Cannula Kientec Systems Cat# FSS-LC-330 Fiber Mating Split Sleeves Precision Fiber Products SM-C5125S Heat Curing Epoxy Precision Fiber Products PFP-353ND Optical Patch Cord Thor Labs Cat# M72L05 Branching Fiberoptic Patch Cord Doric BFP(2)FCM-2×(CL) Tungsten Microelectrodes Microprobes Part# WE3PT30.1B10 Electrode Pellet A-M Systems Cat# 550010 Differential Amplifier Model 1700 A-M Systems Cat# 692000 473 nm Laser Shanghai Lasers BL473T3–100 473 nm Laser IkeCool IKE-PS-500 Laser Power Meter Spectra Physics 407A Two Channel Optical Commutator Doric FRJ 1×2 FC-2FC One Channel Optical Commutator Doric FRJ 1×1 FC-FC Isolated Pulse Stimulator Model 2100 A-M Systems Cat# 720000 Digital Acquisition Board National Instruments BNC-2090 Open in a separate window KEY RESOURCES TABLE Two inputs onto songbird midbrain dopamine neurons evaluate song performance.

Techniques: Plasmid Preparation, Recombinant, Blocking Assay, Software, Isolation